PD-L1 Antikörper (Isoform 1)

Produktnummer ABIN6990335

Der Kaninchen Polyklonal Anti-PD-L1-Antikörper wurde für WB, FACS, ELISA, IF und IHC (p) validiert. Er ist geeignet, PD-L1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für PD-L1 Antikörper (Isoform 1) (ABIN6990335)

Target: PD-L1 (CD274 (PD-L1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PD-L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PD-L1 Antikörper

Bindungsspezifität: AA 60-110, Isoform 1

Spezifität: PD-L1 antibody has no cross-reactivity to PD-L2.

Aufreinigung: PD-L1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-PD-L1 antibody was raised against a peptide corresponding to 17 amino acids near the center of human PD-L1 isoform 1. The immunogen is located within amino acids 60 - 110 of PD-L1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1-2 μ,g/mL, IHC-P: 2.5-5 μ,g/mL, IF: 20 μ,g/mL, Flow Cyt: 0.5 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human and rat samples, Immunofluorescence in human and rat samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PD-L1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu PD-L1

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: PD-L1

Hintergrund: PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3). Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4).

Molekulargewicht: Predicted: 33 kDa

Observed: 37 kDa

Gen-ID: 29126

NCBI Accession: NP_054862

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints