DFFA Antikörper (N-Term)

Produktnummer ABIN6990266

Der Kaninchen Polyklonal Anti-DFFA-Antikörper wurde für WB, ELISA, IF und IHC (p) validiert. Er ist geeignet, DFFA in Proben von Maus zu detektieren.

Kurzübersicht für DFFA Antikörper (N-Term) (ABIN6990266)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DFFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DFFA Antikörper

Bindungsspezifität: N-Term

Aufreinigung: ICAD Antibody is affinity chromatography purified via peptide column.

Immunogen: ICAD antibody was raised against a peptide corresponding to amino acids near the amino terminus of mouse ICAD. The immunogen is located within the first 50 amino acids of ICAD.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ICAD antibody can be used for detection of of ICAD by Western blot at 1 μ,g/mL. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: ICAD Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: ICAD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Andere Bezeichnung: ICAD

Hintergrund: ICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which is cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.

Molekulargewicht: 45 kDa

Gen-ID: 13347

UniProt: O54786

Pathways: Apoptose, Caspase Kaskade in der Apoptose