FLIP Antikörper (N-Term)

Produktnummer ABIN6990260

Produktdetails anti-FLIP Antikörper

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FLIP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF)

Spezifität: FLIP recognizes all FLIP splice variants including FLIPa, b, and y.

Aufreinigung: FLIP Antibody is affinity chromatography purified via peptide column.

Immunogen: FLIP antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP. The sequence is identical in all FLIP splice variants. The immunogen is located within the first 50 amino acids of FLIP.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FLIP antibody can be used for Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL and immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in mouse samples, Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: FLIP Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: FLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Andere Bezeichnung: FLIP (CFLAR Produkte)

Synonyme: Clarp1 antikoerper, cflar antikoerper, CFLAR antikoerper, FLIP antikoerper, CASH antikoerper, CASP8AP1 antikoerper, CLARP antikoerper, Casper antikoerper, FLAME antikoerper, FLAME-1 antikoerper, FLAME1 antikoerper, I-FLICE antikoerper, MRIT antikoerper, c-FLIP antikoerper, c-FLIPL antikoerper, c-FLIPR antikoerper, c-FLIPS antikoerper, 2310024N18Rik antikoerper, A430105C05Rik antikoerper, Cash antikoerper, ENSMUSG00000072980 antikoerper, Flip antikoerper, Gm9845 antikoerper, c-Flip antikoerper, CASP8 and FADD-like apoptosis regulator a antikoerper, CASP8 and FADD like apoptosis regulator antikoerper, CASP8 and FADD like apoptosis regulator S homeolog antikoerper, CASP8 and FADD-like apoptosis regulator antikoerper, cflara antikoerper, CFLAR antikoerper, cflar antikoerper, cflar.S antikoerper, Cflar antikoerper

Hintergrund: FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.

Molekulargewicht: Predicted: 53 kDa

Observed: 53 kDa

Gen-ID: 8837

UniProt: O15519

Pathways: Apoptose, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development