DFFA Antikörper (N-Term)

Produktnummer ABIN6990255

Dieses Anti-DFFA-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von DFFA in WB und ELISA. Geeignet für Human.

Kurzübersicht für DFFA Antikörper (N-Term) (ABIN6990255)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DFFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-DFFA Antikörper

Bindungsspezifität: N-Term

Aufreinigung: DFF45 Antibody is Antibody is DEAE purified.

Immunogen: DFF45 antibody was raised against a 20 amino acid peptide near the amino terminus of human DFF45. The immunogen is located within the first 50 amino acids of DFF45.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: DFF45 antibody can be used for detection of DFF45, DFF35, and one the cleaved fragment by Western blot at 1:1000 to 1:2000 dilution. 45 and 35 kDa bands can be detected in non-apoptotic cells.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: DFF45 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: DFF45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Andere Bezeichnung: DFF45

Hintergrund: DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.

Molekulargewicht: 45 and 35 kDa

Gen-ID: 1676

NCBI Accession: NP_004392

UniProt: O00273

Pathways: Apoptose, Caspase Kaskade in der Apoptose