Endonuclease G Antikörper (AA 51-140)

Produktnummer ABIN6990177

Dieses Anti-Endonuclease G-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Endonuclease G in WB und ELISA. Geeignet für Human, Ratte und Maus.

Kurzübersicht für Endonuclease G Antikörper (AA 51-140) (ABIN6990177)

Target: Endonuclease G (ENDOG)

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Endonuclease G Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 7G1C10

Produktdetails anti-Endonuclease G Antikörper

Bindungsspezifität: AA 51-140

Aufreinigung: EndoG Monoclonal Antibody is Protein A purified.

Immunogen: Recombinant protein corresponding to amino acids 51 - 140 of human EndoG.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: EndoG monoclonal antibody can be used for detection of EndoG by Western blot at 2.5 - 5 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: EndoG Monoclonal Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: EndoG monoclonal antibody can be stored at -20°C, stable for one year.

Details zu Endonuclease G

Target: Endonuclease G (ENDOG)

Andere Bezeichnung: EndoG

Hintergrund: EndoG Monoclonal Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.

Gen-ID: 2021

UniProt: Q14249

Pathways: Apoptose