Rpb1 CTD Antikörper (pSer5)

Produktnummer ABIN6972638

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch Rpb1 CTD in WB, IF und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rpb1 CTD Antikörper (pSer5) (ABIN6972638)

Target: Rpb1 CTD (RNA Polymerase II CTD)

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Rpb1 CTD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 1H4B6

Produktdetails anti-Rpb1 CTD Antikörper

Bindungsspezifität: pSer5

Produktmerkmale: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription. RNA pol II CTD phospho Ser5 antibody (mAb) (Clone 1H4B6) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Andere Bezeichnung: RNA pol II CTD

Molekulargewicht: 240 kDa

NCBI Accession: NP_000928