Nanog Antikörper (N-Term)

Produktnummer ABIN6972403

Dieses Anti-Nanog-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Nanog in WB, IF, ICC, ChIP-seq, ChIP und CUT&RUN. Geeignet für Human und Maus.

Kurzübersicht für Nanog Antikörper (N-Term) (ABIN6972403)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nanog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Produktdetails anti-Nanog Antikörper

Bindungsspezifität: N-Term

Produktmerkmale: Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity). Nanog antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.

Aufreinigung: Affinity Purified

Immunogen: This antibody was raised against a peptide within the N-terminal region of mouse Nanog.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: Nanog

Molekulargewicht: 45 kDa

NCBI Accession: NP_082292

Pathways: Stem Cell Maintenance