IL-21 Antikörper

Produktnummer ABIN6964031

Produktdetails anti-IL-21 Antikörper

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-21 Antikörper ist unkonjugiert

Applikation: Neutralization (Neut)

Spezifität: Native and recombinant IL-21

Aufreinigung: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-21

Klon: MT216G-21-3m

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Neutralization of human IL-21 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: The neutralizing capacity and ND50 was determined using ELISA and ELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/mL) with or without serial dilutions of mAbs MT216G/21.3m. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/mL) with or without serial dilutions of mAbs MT216G/21.3m. After 4 days the IgG-secreting B cells were quantified by ELISpot. Neutralization dose 50 (ND50): MT216G/21.3m neutralized the effect of IL-21 at a ND50value of approximately 0.05 μg/mL when HEK cells were activated with 10 ng/mL of IL-21 (A). The corresponding ND50value for human PBMC activated with 10 ng/mL IL-21 was approximately 0.2 μg/mL. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS free of preservatives

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Haltbarkeit: 18 months

Referenzen für anti-IL-21 Antikörper (ABIN6964031)

You, Zhang, Cui, Zhu, Lv, Tang, Sun: "Th9 cells promote antitumor immunity via IL-9 and IL-21 and demonstrate atypical cytokine expression in breast cancer." in: International immunopharmacology, Vol. 52, pp. 163-167, (2018) (PubMed).

Huang, Ehrnfelt, Paulie, Zuber, Ahlborg: "ELISpot and ELISA analyses of human IL-21-secreting cells: Impact of blocking IL-21 interaction with cellular receptors." in: Journal of immunological methods, Vol. 417, pp. 60-66, (2015) (PubMed).

Details zu IL-21

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Andere Bezeichnung: IL21 (IL21 Produkte)

Synonyme: IL-21 antikoerper, Za11 antikoerper, interleukin 21 antikoerper, IL21 antikoerper, Il21 antikoerper

Gen-ID: 59067

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response