Interferon gamma Antikörper (PromoFluor 647 Premium)

Produktnummer ABIN6963791

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Interferon gamma in FACS. Er zeigt eine Reaktivität gegenüber Schwein.

Kurzübersicht für Interferon gamma Antikörper (PromoFluor 647 Premium) (ABIN6963791)

Target: Interferon gamma (IFNG)

Reaktivität: Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Interferon gamma Antikörper ist konjugiert mit PromoFluor 647 Premium

Applikation: Flow Cytometry (FACS)

Klon: P2C11

Produktdetails anti-Interferon gamma Antikörper (PromoFluor 647 Premium)

Spezifität: Native and recombinant porcine IFN-γ

Aufreinigung: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant porcine IFN-gamma

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable porcine PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with CD3-PE and P2C11-PF647P (right panel). The left panel depicts cells stained with a matched isotype control antibody. The dot plots derive from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Haltbarkeit: 18 months

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFNG

Gen-ID: 396991

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie