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Interferon gamma Antikörper (PromoFluor-488 Premium)

Dieses Anti-Interferon gamma-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Interferon gamma in FACS. Geeignet für Meerschweinchen.
Produktnummer ABIN6963784

Kurzübersicht für Interferon gamma Antikörper (PromoFluor-488 Premium) (ABIN6963784)

Target

Alle Interferon gamma (IFNG) Antikörper anzeigen
Interferon gamma (IFNG)

Reaktivität

  • 277
  • 104
  • 68
  • 31
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  • 24
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Meerschweinchen

Wirt

  • 287
  • 223
  • 48
  • 12
  • 11
  • 4
  • 2
  • 2
  • 1
  • 1
Maus

Klonalität

  • 344
  • 242
  • 3
Monoklonal

Konjugat

  • 287
  • 99
  • 54
  • 22
  • 20
  • 15
  • 7
  • 5
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  • 4
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  • 4
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  • 2
  • 2
  • 2
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  • 2
  • 1
  • 1
  • 1
  • 1
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Dieser Interferon gamma Antikörper ist konjugiert mit PromoFluor-488 Premium

Applikation

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Flow Cytometry (FACS)

Klon

MT4A27
  • Spezifität

    Native and recombinant IFN-γ

    Aufreinigung

    Purified from in vitro cultures by protein G affinity chromatography.

    Immunogen

    Recombinant guinea pig IFN-gamma

    Isotyp

    IgG1
  • Applikationshinweise

    Detection of guinea pig IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. PF488P is excited by the blue laser (488 nm). The excitation max is 490 nm and the emission max is 516 nm. Detection of IFN-γ by flow cytometry in viable guinea pig spleen cells. Cells were stimulated for 16 hrs in the presence of PMA/ ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MT4A27-PF488P (solid line). Matched isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    in PBS with 0.2 % BSA and 0.09 % sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

    Haltbarkeit

    18 months
  • Target

    Interferon gamma (IFNG)

    Andere Bezeichnung

    IFNG

    Gen-ID

    100379568

    Pathways

    Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie
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