Interferon gamma Antikörper (PromoFluor 647 Premium)

Produktnummer ABIN6963781

Dieses Anti-Interferon gamma-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Interferon gamma in FACS. Geeignet für Hispid Cotton Rat.

Kurzübersicht für Interferon gamma Antikörper (PromoFluor 647 Premium) (ABIN6963781)

Target: Interferon gamma (IFNG)

Reaktivität: Hispid Cotton Rat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Interferon gamma Antikörper ist konjugiert mit PromoFluor 647 Premium

Applikation: Flow Cytometry (FACS)

Klon: MT417

Produktdetails anti-Interferon gamma Antikörper (PromoFluor 647 Premium)

Spezifität: Native and recombinant IFN-γ

Aufreinigung: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant cotton rat IFN-gamma

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Detection of cotton rat IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable cotton rat spleen cells. Cells were stimulated for 16 hours in the presence of PMA/ ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MT417-PF647P (solid line). Matched isotype control antibody (dashed line). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Haltbarkeit: 18 months

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFNG

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie