Interferon gamma Antikörper (PromoFluor 647 Premium)

Produktnummer ABIN6963777

Der Maus Monoklonal Anti-Interferon gamma-Antikörper wurde für FACS validiert. Er ist geeignet, Interferon gamma in Proben von Rind (Kuh), Schaf und Ziege zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Interferon gamma Antikörper (PromoFluor 647 Premium) (ABIN6963777)

Target: Interferon gamma (IFNG)

Reaktivität: Rind (Kuh), Schaf, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Interferon gamma Antikörper ist konjugiert mit PromoFluor 647 Premium

Applikation: Flow Cytometry (FACS)

Klon: MT307

Produktdetails anti-Interferon gamma Antikörper (PromoFluor 647 Premium)

Spezifität: Native and recombinant IFN-γ.

Kreuzreaktivität (Details): The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.

Aufreinigung: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant bovine IFN-gamma

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable bovine PBMC. Cells were stimulated for 16 hours in the presence of PHA and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MT307-PF647P (left panel). The specificity of MT307-PF647P was demonstrated by pre-incubating the antibody with recombinant bovine IFN-γ prior to staining of PBMC (middle panel). Matched isotype control antibody (right panel). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Haltbarkeit: 18 months

Referenzen für anti-Interferon gamma Antikörper (PromoFluor 647 Premium) (ABIN6963777)

Richt, Kasinathan, Hamir, Castilla, Sathiyaseelan, Vargas, Sathiyaseelan, Wu, Matsushita, Koster, Kato, Ishida, Soto, Robl, Kuroiwa: "Production of cattle lacking prion protein." in: Nature biotechnology, Vol. 25, Issue 1, pp. 132-8, (2007) (PubMed).

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFNG

Gen-ID: 281237, 443396

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie