Interferon gamma Antikörper (FITC)

Produktnummer ABIN6963776

Dieser Maus Monoklonal Antikörper erkennt spezifisch Interferon gamma in FACS. Er zeigt eine Reaktivität gegenüber Rind (Kuh), Schaf und Ziege.

Kurzübersicht für Interferon gamma Antikörper (FITC) (ABIN6963776)

Target: Interferon gamma (IFNG)

Reaktivität: Rind (Kuh), Schaf, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Interferon gamma Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: MT307

Produktdetails anti-Interferon gamma Antikörper (FITC)

Spezifität: Native and recombinant IFN-γ.

Kreuzreaktivität (Details): The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.

Aufreinigung: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant bovine IFN-gamma

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in bovine peripheral blood mononuclear cells (PBMC). Bovine PBMC were stimulated with PMA/ionomycin for 16 hours in the presence of Brefeldin A. The cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti-bovine IFN-γ mAb MT307-FITC (right panel). The left panel depicts stimulated cells stained with a matched isotype control antibody. The dot plots derive from a lymphocyte gate. Flow cytometry was performed on a Guava EasyCyte Mini.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Haltbarkeit: 18 months

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFNG

Gen-ID: 281237, 443396

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie