IL-2 Antikörper (PromoFluor 647 Premium)

Produktnummer ABIN6963756

Der Maus Monoklonal Anti-IL-2-Antikörper wurde für FACS validiert. Er ist geeignet, IL-2 in Proben von Rind (Kuh) und Schaf zu detektieren.

Kurzübersicht für IL-2 Antikörper (PromoFluor 647 Premium) (ABIN6963756)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reaktivität: Rind (Kuh), Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-2 Antikörper ist konjugiert mit PromoFluor 647 Premium

Applikation: Flow Cytometry (FACS)

Klon: MT3B3

Produktdetails anti-IL-2 Antikörper (PromoFluor 647 Premium)

Spezifität: Native and recombinant IL-2

Kreuzreaktivität (Details): The monoclonal antibody cross-reacts with IL-2 from sheep. For dection of IL-2 in sheep, unconjugated MT3B3 with second step detection is recommended.

Aufreinigung: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant bovine IL-2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Detection of IL-2 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-2 by flow cytometry in viable bovine PBMC. Cells were stimulated for 5 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti- bovine IL-2 mAb MT3B3-PF647P (solid line). Matched isotype control antibody MT128. The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Haltbarkeit: 18 months

Details zu IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Andere Bezeichnung: IL2

Gen-ID: 280822

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation