CD8 alpha Antikörper

Produktnummer ABIN6961722

Der Maus Monoklonal anti-CD8 alpha Antikörper wird verwendet zum Nachweis von CD8 alpha in Proben von Human. Er wurde validiert für FACS, IP und IHC (fro).

Kurzübersicht für CD8 alpha Antikörper (ABIN6961722)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD8 alpha Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: RPA-T8

Produktdetails anti-CD8 alpha Antikörper

Kreuzreaktivität: Pavian, Schimpanse, Cynomolgus, Rhesusaffen, Sooty mangabey (Cercocebus atys)

Aufreinigung: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Kommentare:

0.5 mg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C

Haltbarkeit: 12 months

Details zu CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: CD8a

Hintergrund: The RPA-T8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a ho- modimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone Hit8a, the RPA-T8 antibody will not block binding of Hit8a to CD8a.

Gen-ID: 925

UniProt: P01732

Pathways: T-Zell Rezeptor Signalweg