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Rekombinanter SARS-CoV-2 Spike S1 Antikörper (RBD)

Dieses Anti-SARS-CoV-2 Spike S1-Antikörper ist ein Human Monoclonal-Antikörper zur Detektion von SARS-CoV-2 Spike S1 in ELISA, GICA, Neut. Geeignet für SARS Coronavirus-2 (SARS-CoV-2).
Produktnummer ABIN6953152

Kurzübersicht für Rekombinanter SARS-CoV-2 Spike S1 Antikörper (RBD) (ABIN6953152)

Target

Alle SARS-CoV-2 Spike S1 Antikörper anzeigen
SARS-CoV-2 Spike S1

Antikörpertyp

Recombinant Antibody

Fragment

single-domain Antibody (sdAb)

Reaktivität

  • 45
  • 6
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
SARS Coronavirus-2 (SARS-CoV-2)

Wirt

  • 17
  • 10
  • 9
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Klonalität

  • 33
  • 8
  • 5
Monoklonal

Konjugat

  • 40
  • 3
  • 1
  • 1
  • 1
Dieser SARS-CoV-2 Spike S1 Antikörper ist unkonjugiert

Applikation

  • 46
  • 10
  • 8
  • 6
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
ELISA, Colloidal Gold Immunochromatography Assay (GICA), Neutralization (Neut)

Klon

A1
  • Bindungsspezifität

    • 12
    • 3
    • 2
    • 2
    • 1
    RBD

    Produktmerkmale

    This SARS-CoV-2 Spike RBD Nanobody is a recombinant monoclonal antibody generated through the expression of a DNA sequence inserting a human IgG1 Fc domain at the C-terminus, in human embryonic kidney 293 cells (HEK293). The DNA sequence encodes the SARS-CoV-2 spike receptor-binding domain (RBD). The antibody is purified by protein G in vitro. It has been validated with high reactivity towards SARS-CoV-2-S1-RBD by a functional ELISA and good sensitivity for human SARS-CoV-2 spike glycoprotein (S protein) via the Colloidal Gold Immunochromatography Assay (GICA). In neutralization assay, the binding signal of SARS-CoV-2 Spike RBD Nanobody was inhibited by ACE2 protein-HRP conjugated inhibitor, with a 0.1074 μg/mL IC50. Specifically binding and recognizing the RBD of the SARS-CoV-2 spike glycoprotein (S protein), so the SARS-CoV-2 Spike RBD Nanobody can react with samples infected with human coronavirus SARS-CoV-2. But it does not respond to MERS or SARS-CoV spike protein. Akin to other nanobodies, this recombinant nanobody is small and stable, which allows for its reaching to hidden epitopes such as crevices of target proteins.
    VHH fusion with human IgG1 Fc

    Aufreinigung

    affinity-chromatography

    Immunogen

    Recombinant Human Novel Coronavirus Spike glycoprotein(S) (319-541aa)

    Isotyp

    IgG1
  • Applikationshinweise

    ELISA 1:10000-1:100000
    GICA 1:10000-1:40000
    Neutralising 1:100-1:10000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    50 % Glycerol, 0.01M PBS, pH 7.4, 0.03 % Proclin 300

    Konservierungsmittel

    ProClin

    Vorsichtsmaßnahmen

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C,-80 °C

    Informationen zur Lagerung

    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze
  • Target

    SARS-CoV-2 Spike S1

    Substanzklasse

    Viral Protein

    Hintergrund

    Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells.

    Gen-ID

    43740568

    UniProt

    P0DTC2
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