Prohibitin Antikörper

Produktnummer ABIN6944678

Dieses Anti-Prohibitin-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von Prohibitin in WB, FACS, IHC (p), IF (cc) und IHC (fro). Geeignet für Human, Maus, Ratte, Zebrafisch (Danio rerio), Crucian Carp und Fisch.

Kurzübersicht für Prohibitin Antikörper (ABIN6944678)

Target: Prohibitin (PHB)

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Crucian Carp, Fisch

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Prohibitin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 1F10

Produktdetails anti-Prohibitin Antikörper

Kreuzreaktivität: Fisch, Human, Maus, Ratte, Zebrafisch (Danio rerio)

Kreuzreaktivität (Details): Crucian carp (Carassius carassius)

Aufreinigung: Purified by Protein A.

Immunogen: N terminal Human Prohibitin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu Prohibitin

Target: Prohibitin (PHB)

Andere Bezeichnung: Prohibitin

Hintergrund:

Synonyms: Prohibitin, PHB

Background: Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity, and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progressionin more than 80 % of the cell lines analyzed.

Gen-ID: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling