MEK1/2 Antikörper

Produktnummer ABIN6944005

Der Kaninchen Monoklonal anti-MEK1/2 Antikörper wird verwendet zum Nachweis von MEK1/2 in Proben von Human, Maus, Ratte, Zebrafisch (Danio rerio), Crucian Carp und Fisch. Er wurde validiert für WB, IF (cc), IF (p), IHC (p) und FACS.

Kurzübersicht für MEK1/2 Antikörper (ABIN6944005)

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Crucian Carp, Fisch

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MEK1/2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Klon: 4H7

Produktdetails anti-MEK1/2 Antikörper

Spezifität: The antibody will detect both MAP2K1 and MAP2K2

Kreuzreaktivität: Fisch, Human, Maus, Ratte, Zebrafisch (Danio rerio)

Kreuzreaktivität (Details): Crucian carp (Carassius carassius)

Aufreinigung: Purified by Protein A.

Immunogen: Human MAP2K1 + MAP2K2 aa 1 to the C-terminus

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu MEK1/2

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Andere Bezeichnung: MAP2K1/2

Hintergrund:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1, MAP2K1, MEK1, MKK2, PRKMK1, MAPKK1, Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAPK/ERK kinase 2, MAP2K2, MEK2, MKK2, PRKMK2, MAPKK2

Background: A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

Gen-ID: 5604, 5605

UniProt: Q02750, P36507