CD3D Antikörper

Produktnummer ABIN6942432

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch CD3D in WB, IHC, FACS, IHC (p) und IF (cc). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD3D Antikörper (ABIN6942432)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD3D Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Klon: 5C1

Produktdetails anti-CD3D Antikörper

Kreuzreaktivität: Human

Aufreinigung: Purified by Protein A.

Immunogen: C terminus human CD3D

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Andere Bezeichnung: CD3D

Hintergrund:

Synonyms: T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD_antigen: CD3d, CD3D

Background: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456).

Gen-ID: 915

UniProt: P04234

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events