Rekombinanter KRT77, KRT76 Antikörper

Produktnummer ABIN6941457

Der Kaninchen Monoklonal anti-KRT77, KRT76 Antikörper (Klon KRTH-1576R-KRTL-1577R) (ABIN6941457) detektiert spezifisch KRT77, KRT76 in IHC und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Hund, Maus, Kaninchen und Meerschweinchen.

Kurzübersicht für Rekombinanter KRT77, KRT76 Antikörper (ABIN6941457)

Target: KRT77, KRT76

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Hund, Maus, Kaninchen, Meerschweinchen

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Klon: KRTH-1576R-KRTL-1577R

Produktdetails

Verwendungszweck: Recombinant Cytokeratin, pan (Epithelial Marker) Antibody

Spezifität: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas.

Kreuzreaktivität (Details): Expected to show broad species reactivity.

Aufreinigung: 200ug/ml of recombinant MAb Purified by Protein A/G.

Immunogen: Recombinant full-length human KRT76 and KRT77 proteins

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Skin, Adeno- or Squamous carcinomas.

Known Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: KRT77, KRT76

Andere Bezeichnung: KRT77 & KRT76

Hintergrund:

K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Adeno- or Squamous carcinomas., Skin, A431

Molekulargewicht: 40-67kDa

Gen-ID: 374454, 51350, 334989

UniProt: Q7Z794, Q01546