KRT Antikörper

Produktnummer ABIN6941421

Der Maus Monoklonal anti-KRT Antikörper wird verwendet zum Nachweis von KRT in Proben von Human, Cat und Hund. Er wurde validiert für IHC, FACS und IF.

Kurzübersicht für KRT Antikörper (ABIN6941421)

Target: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Reaktivität: Human, Cat, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: PCK-3150

Produktdetails anti-KRT Antikörper

Verwendungszweck: Cytokeratin, pan (Epithelial Marker) Antibody

Spezifität: Anti-cytokeratin clone PCK/3150 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/3150 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/3150 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Skin, adeno- or squamous carcinomas (IHC).

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu KRT

Target: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Andere Bezeichnung: KRT

Hintergrund:

K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

adeno- or squamous carcinomas (IHC)., Skin

Molekulargewicht: 40-67kDa ()