ACTA1/ACTA2A/ACTG2 Antikörper
Kurzübersicht für ACTA1/ACTA2A/ACTG2 Antikörper (ABIN6941399)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
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Aufreinigung
- Purified by Protein A/G
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Immunogen
- Synthetic peptide common among human muscle actins
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Isotyp
- IgG1 kappa
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anti-ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth) antibody
Reaktivität: Human, Kaninchen, Ratte, Cat, Huhn, Hund, Maus FACS, IF, IHC, StM Wirt: Maus Monoclonal HHF35 unconjugated
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Applikationshinweise
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Positive Control: Muscle or sarcoma.
Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Haltbarkeit
- 24 months
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