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ACTA1/ACTA2A/ACTG2 Antikörper

Reaktivität: Human FACS, IF, IHC (f) Wirt: Maus Monoclonal HHF35 unconjugated
Produktnummer ABIN6936350
  • Target Alle ACTA1/ACTA2A/ACTG2 Produkte
    ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
    Reaktivität
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 4
    Maus
    Klonalität
    • 4
    Monoklonal
    Konjugat
    • 4
    Dieser ACTA1/ACTA2A/ACTG2 Antikörper ist unkonjugiert
    Applikation
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Spezifität
    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
    Kreuzreaktivität (Details)
    Human. Rabbit. Cat. Dog. Mouse. Rat. Chicken.
    Aufreinigung
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    SDS extract of human myocardium.
    Klon
    HHF35
    Isotyp
    IgG1 kappa
  • Applikationshinweise

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Muscle or sarcoma.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Konservierungsmittel
    Azide free
    Lagerung
    -20 °C,-80 °C
    Informationen zur Lagerung
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Haltbarkeit
    24 months
  • Target
    ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
    Abstract
    ACTA1/ACTA2A/ACTG2 Produkte
    Hintergrund
    ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1,Actin, Muscle Specific (Muscle Cell Marker)
    Cellular localisation: Cytoplasmic
    Molekulargewicht
    Multiple
    Gen-ID
    58, 59, 70
    UniProt
    P62736, P68032, P68133
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