BrdU Antikörper

Produktnummer ABIN6941366

Der Maus Monoklonal Anti-BrdU-Antikörper wurde für IHC, FACS, IF, Neut und StM validiert. Er ist geeignet, BrdU in Proben von zu detektieren.

Kurzübersicht für BrdU Antikörper (ABIN6941366)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BrdU Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Neutralization (Neut), Staining Methods (StM)

Klon: SPM166

Produktdetails anti-BrdU Antikörper

Spezifität: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Aufreinigung: Purified by Protein A/G

Immunogen: Bromodeoxyuridine (BrdU) conjugated to KLH

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Use Ab at 1 μg/mL for 30 minute at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Substanzklasse: Chemical

Molekulargewicht: 307Da