CDC20 Antikörper

Produktnummer ABIN6941283

Der Maus Monoklonal Anti-CDC20-Antikörper wurde für FACS, IF, IHC und StM validiert. Er ist geeignet, CDC20 in Proben von Human zu detektieren.

Kurzübersicht für CDC20 Antikörper (ABIN6941283)

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDC20 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: AR12

Produktdetails anti-CDC20 Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Urea-denatured His6 Cdc20 human recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Ramos or HeLa cells. Tonsil or gastric carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CDC20

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Andere Bezeichnung: CDC20

Hintergrund: Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.

Molekulargewicht: 55kDa

Gen-ID: 991

UniProt: Q12834