CD53 Antikörper

Produktnummer ABIN6941219

Der Maus Monoklonal anti-CD53 Antikörper wird verwendet zum Nachweis von CD53 in Proben von Human. Er wurde validiert für FACS, IF und Func.

Kurzübersicht für CD53 Antikörper (ABIN6941219)

Target: CD53

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD53 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Klon: 161-2

Produktdetails anti-CD53 Antikörper

Verwendungszweck: CD53 (TSPAN25) Antibody

Spezifität: Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI, Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

Keine Kreuzreaktivität: Pavian, Pferd

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Stimulated human leukocytes

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.

Known Application: Functional Studies (Order Ab without Azide),Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD53

Target: CD53

Andere Bezeichnung: CD53

Hintergrund:

CD53 glycoprotein, CD53 tetraspan antigen, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, Tetraspanin-25 (Tspan-25), TSPAN25

Daudi, HL-60, human leukocytes. Human tonsil and lymph node., HUT-102, HUT-78, IM9, Jurkat, Kg1a, Raji, THP-1, U266, YT

Molekulargewicht: 33-55kDa

Gen-ID: 963, 443057

UniProt: P19397