CD47 Antikörper

Produktnummer ABIN6941213

Der Maus Monoklonal Anti-CD47-Antikörper wurde für FACS, IHC, IF, Func und StM validiert. Er ist geeignet, CD47 in Proben von Human und Maus zu detektieren.

Kurzübersicht für CD47 Antikörper (ABIN6941213)

Target: CD47

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD47 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func), Staining Methods (StM)

Klon: IAP-964-B6H12-2

Produktdetails anti-CD47 Antikörper

Spezifität: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human IAP protein (IAP/964), Intact CD47 Purified from placenta (B6H12.2)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7, HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors.

Known Application: Functional Studies (Order Ab without Azide), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD47

Target: CD47

Andere Bezeichnung: CD47

Molekulargewicht: ~50kDa

Gen-ID: 961

UniProt: Q08722