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CD47 Antikörper

Dieses Anti-CD47-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD47 in FACS, IF, IHC und StM. Geeignet für Human und Maus.
Produktnummer ABIN6941211

Kurzübersicht für CD47 Antikörper (ABIN6941211)

Target

Alle CD47 Antikörper anzeigen
CD47

Reaktivität

  • 132
  • 52
  • 26
  • 7
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Maus

Wirt

  • 79
  • 70
  • 9
  • 4
  • 1
Maus

Klonalität

  • 89
  • 69
  • 4
Monoklonal

Konjugat

  • 77
  • 13
  • 11
  • 7
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CD47 Antikörper ist unkonjugiert

Applikation

  • 101
  • 85
  • 46
  • 43
  • 39
  • 26
  • 26
  • 19
  • 18
  • 15
  • 13
  • 12
  • 9
  • 6
  • 5
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Klon

B6H12-2
  • Spezifität

    This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Intact CD47 Purified from placenta

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    Positive Control: MCF-7, HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    CD47

    Andere Bezeichnung

    CD47

    Molekulargewicht

    ~50kDa

    Gen-ID

    961

    UniProt

    Q08722
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