CD8 alpha Antikörper

Produktnummer ABIN6941072

Der Maus Monoklonal anti-CD8 alpha Antikörper wird verwendet zum Nachweis von CD8 alpha in Proben von Human. Er wurde validiert für FACS.

Kurzübersicht für CD8 alpha Antikörper (ABIN6941072)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD8 alpha Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: UCHT4

Produktdetails anti-CD8 alpha Antikörper

Verwendungszweck: CD8A (Cytotoxic- & Suppressor T-Cell Marker) Antibody

Spezifität: CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen. This MAb binds to the CD8 alpha chain, at an epitope distinct from that recognized by UCHT14.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Thymocytes followed by Sezary T cells.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat, HuT78 or hPBL. Tonsil.

Known Application: Flow Cytometry (1-2 μg/million cells), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: CD8A

Hintergrund:

CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain

HuT78 or hPBL. Tonsil., Jurkat

Molekulargewicht: 32kDa

Gen-ID: 925, 85258

UniProt: P01732

Pathways: T-Zell Rezeptor Signalweg