Rekombinanter Aurora Kinase B Antikörper (AA 89-251)

Produktnummer ABIN6941049

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch Aurora Kinase B in IHC und StM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter Aurora Kinase B Antikörper (AA 89-251) (ABIN6941049)

Target: Aurora Kinase B (AURKB)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Aurora Kinase B Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: AURKB-3121R

Produktdetails anti-Aurora Kinase B Antikörper

Bindungsspezifität: AA 89-251

Spezifität: Recognizes a protein of 39 kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Tonsil, Placenta, Hodgkin's Lymphoma or Colon Carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Andere Bezeichnung: AURKB

Molekulargewicht: 39kDa

Gen-ID: 9212

UniProt: Q96GD4

Pathways: T-Zell Rezeptor Signalweg, Zellzyklus, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades