MAML2 Antikörper

Produktnummer ABIN6940961

Der Maus Monoklonal Anti-MAML2-Antikörper wurde für WB, IF, IHC, FACS, ISt und StM validiert. Er ist geeignet, MAML2 in Proben von Human zu detektieren.

Kurzübersicht für MAML2 Antikörper (ABIN6940961)

Target: MAML2 (Mastermind-Like 2 (MAML2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAML2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)

Klon: MAML2-1302

Produktdetails anti-MAML2 Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human MAML2 fragment

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MAML2

Target: MAML2 (Mastermind-Like 2 (MAML2))

Andere Bezeichnung: MAML2

Hintergrund: MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.

Molekulargewicht: 125kDa

Gen-ID: 84441

UniProt: Q8IZL2