XRCC3 Antikörper

Produktnummer ABIN6940907

Dieses Anti-XRCC3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von XRCC3 in WB, IHC und StM. Geeignet für Human.

Kurzübersicht für XRCC3 Antikörper (ABIN6940907)

Target: XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser XRCC3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: 10F1-6

Produktdetails anti-XRCC3 Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human XRCC3 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human placenta. HeLa nuclear extract.

Known Application: Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu XRCC3

Target: XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3))

Andere Bezeichnung: XRCC3

Hintergrund: The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA Ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA Ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA Ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.

Molekulargewicht: 40kDa

Gen-ID: 7517

UniProt: O43542

Pathways: DNA Reparatur