Vimentin Antikörper
Kurzübersicht für Vimentin Antikörper (ABIN6940881)
Target
Alle Vimentin (VIM) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Vimentin (Mesenchymal Cell Marker) Antibody
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Spezifität
- This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.
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Aufreinigung
- 200ug/ml of Ab Purified from Bioreactor Concentrate.
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Immunogen
- Human thymic nuclear extract
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Isotyp
- IgM, kappa
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Applikationshinweise
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Positive Control: Jurkat cells, Sarcomas, Melanomas.
Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Haltbarkeit
- 24 months
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- Vimentin (VIM)
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Andere Bezeichnung
- VIM
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Hintergrund
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VIM
Jurkat cells, Melanomas., Sarcomas
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Molekulargewicht
- 57-60kDa
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Gen-ID
- 7431, 455493
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UniProt
- P08670
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Pathways
- Caspase Kaskade in der Apoptose
Target
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