Villin 1 Antikörper (AA 179-311)

Produktnummer ABIN6940871

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Villin 1 in IHC, WB und StM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Villin 1 Antikörper (AA 179-311) (ABIN6940871)

Target: Villin 1 (VIL1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Villin 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Staining Methods (StM)

Klon: VIL1-1314

Produktdetails anti-Villin 1 Antikörper

Bindungsspezifität: AA 179-311

Spezifität: Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.

Known Application: Western Blot (1-2 μg/mL for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Villin 1

Target: Villin 1 (VIL1)

Andere Bezeichnung: VIL1

Molekulargewicht: 93kDa

Gen-ID: 7429

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization