Vinculin Antikörper (AA 174-322)

Produktnummer ABIN6940865

Der Maus Monoklonal anti-Vinculin Antikörper (Klon VCL-2573) (ABIN6940865) detektiert spezifisch Vinculin in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für Vinculin Antikörper (AA 174-322) (ABIN6940865)

Target: Vinculin (VCL)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vinculin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: VCL-2573

Produktdetails anti-Vinculin Antikörper

Bindungsspezifität: AA 174-322

Verwendungszweck: Vinculin (Marker of Age-related Macular Degeneration) Antibody

Spezifität: Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin each contain Actin binding sites. Reportedly, vinculin is a potential plasma biomarker for Age-related Macular Degeneration (AMD). The early detection of AMD using novel plasma biomarkers with genetic modeling may enable timely treatment and vision preservation in the elderly.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human Bladder and Testis.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Vinculin

Target: Vinculin (VCL)

Andere Bezeichnung: VCL

Hintergrund:

CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, VCL, VINC

Human Bladder and Testis.

Molekulargewicht: 117kDa

Gen-ID: 7414, 643896

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2