Transferrin Receptor Antikörper (AA 94-212)
Kurzübersicht für Transferrin Receptor Antikörper (AA 94-212) (ABIN6940699)
Target
Alle Transferrin Receptor (TFRC) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 94-212
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Spezifität
- It recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
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Aufreinigung
- Purified by Protein A/G
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Immunogen
- Recombinant extracellular fragment (around aa 94-212) of human TFRC protein (exact sequence is proprietary)
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Isotyp
- IgG2b kappa
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Applikationshinweise
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Positive Control: Jurkat, HeLa, MCF-7 or K562 cells. Human placenta or Bone Marrow.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Western Blot (0.5-1.0 μg/mL)Optimal dilution for a specific application should be determined.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Haltbarkeit
- 24 months
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- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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Andere Bezeichnung
- TFRC
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Molekulargewicht
- 85-95kDa (monomer), 190kDa (dimer)
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Gen-ID
- 7037
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UniProt
- P02786
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Pathways
- Transition Metal Ion Homeostasis
Target
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