Spectrin beta Chain, Erythrocyte (SPTB) Antikörper

Produktnummer ABIN6940649

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in WB, IF, IHC, ELISA, Coat und StM. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für Spectrin beta Chain, Erythrocyte (SPTB) Antikörper (ABIN6940649)

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Klon: RG-26

Produktdetails

Aufreinigung: Purified by Protein A/G

Immunogen: Red cell membranes

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Andere Bezeichnung: SPTB

Hintergrund: Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

Molekulargewicht: 246kDa

Gen-ID: 6710

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization