Spectrin beta Chain, Erythrocyte (SPTB) Antikörper

Produktnummer ABIN6938442

Der Maus Monoklonal anti-Spectrin beta Chain, Erythrocyte Antikörper wird verwendet zum Nachweis von Spectrin beta Chain, Erythrocyte in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF und ELISA.

Kurzübersicht für Spectrin beta Chain, Erythrocyte (SPTB) Antikörper (ABIN6938442)

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Klon: RG-26

Produktdetails

Verwendungszweck: Spectrin beta I (SPTB) Antibody

Spezifität: Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

Kreuzreaktivität (Details): Human. Mouse. Rat.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Red cell membranes

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Andere Bezeichnung: SPTB

Hintergrund:

beta-I spectrin, EL3, erythrocyte, HS2, SPTB1, HSPTB1, membrane cytoskeletal protein, spectrin beta (erythrocytic)

Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Molekulargewicht: 246kDa

Gen-ID: 6710, 26915

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization