Spastin Antikörper

Produktnummer ABIN6940629

Der Maus Monoklonal Anti-Spastin-Antikörper wurde für WB, ELISA, IHC, IF, Coat und StM validiert. Er ist geeignet, Spastin in Proben von Maus, Ratte und Human zu detektieren.

Kurzübersicht für Spastin Antikörper (ABIN6940629)

Target: Spastin (SPAST)

Reaktivität: Maus, Ratte, Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Spastin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

Klon: Sp 3G11-1

Produktdetails anti-Spastin Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human Spastin protein.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Rat brain extract.

Known Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Spastin

Target: Spastin (SPAST)

Andere Bezeichnung: SPAST

Hintergrund: The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs.

Molekulargewicht: 52kDa

Gen-ID: 6683

Pathways: Microtubule Dynamics, M Phase, Regulation of Cell Size