Rekombinanter SOX10 Antikörper (AA 115-269)

Produktnummer ABIN6940628

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch SOX10 in IHC und StM. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Rekombinanter SOX10 Antikörper (AA 115-269) (ABIN6940628)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser SOX10 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: SOX10-2311R

Produktdetails anti-SOX10 Antikörper

Bindungsspezifität: AA 115-269

Aufreinigung: Purified by Protein A/G

Immunogen: A recombinant fragment (around aa115-269) of human SOX10 protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: HepG2 cells. Melanomas, breast carcinomas, gliomas.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Andere Bezeichnung: SOX10

Hintergrund: SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Molekulargewicht: 49-58kDa

Gen-ID: 6663

UniProt: P56693

Pathways: Chromatin Binding