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SOX10 Antikörper (AA 115-269)

Der Maus Monoklonal Anti-SOX10-Antikörper wurde für WB, FACS, IF, IHC und StM validiert. Er ist geeignet, SOX10 in Proben von Human zu detektieren.
Produktnummer ABIN6940626

Kurzübersicht für SOX10 Antikörper (AA 115-269) (ABIN6940626)

Target

Alle SOX10 Antikörper anzeigen
SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Reaktivität

  • 83
  • 51
  • 36
  • 6
  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 67
  • 37
  • 2
Maus

Klonalität

  • 56
  • 50
Monoklonal

Konjugat

  • 56
  • 8
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser SOX10 Antikörper ist unkonjugiert

Applikation

  • 84
  • 38
  • 36
  • 31
  • 29
  • 17
  • 15
  • 13
  • 12
  • 10
  • 6
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Klon

SPM607
  • Bindungsspezifität

    • 33
    • 16
    • 8
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 115-269

    Spezifität

    Recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Recombinant fragment (aa 115-269) of human SOX10 protein with hexa-histidine tag

    Isotyp

    IgG2b kappa
  • Applikationshinweise

    Positive Control: HepG2 cells. Melanomas, breast carcinomas, gliomas.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

    Andere Bezeichnung

    SOX10

    Molekulargewicht

    49-58kDa

    Gen-ID

    6663

    UniProt

    P56693

    Pathways

    Chromatin Binding
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