S100A1 Antikörper

Produktnummer ABIN6940539

Der Maus Monoklonal Anti-S100A1-Antikörper wurde für IHC und StM validiert. Er ist geeignet, S100A1 in Proben von Human zu detektieren.

Kurzübersicht für S100A1 Antikörper (ABIN6940539)

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: S100A1-1942

Produktdetails anti-S100A1 Antikörper

Spezifität: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human full-length S100A1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431 or HT-29 cells. Brain or Melanoma.

Known Application: Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Andere Bezeichnung: S100A1

Molekulargewicht: 10-12kDa

Gen-ID: 6271

UniProt: P23297

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteine