BCL2L1 Antikörper (AA 3-14)

Produktnummer ABIN6940510

Dieses Anti-BCL2L1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von BCL2L1 in WB, IHC, IF, FACS und StM. Geeignet für Human, Maus, Ratte und Schwein.

Kurzübersicht für BCL2L1 Antikörper (AA 3-14) (ABIN6940510)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BCL2L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: 2H12

Produktdetails anti-BCL2L1 Antikörper

Bindungsspezifität: AA 3-14

Spezifität: Recognizes a protein of 27 kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.

Aufreinigung: Purified by Protein A/G

Immunogen: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Andere Bezeichnung: BCL2L1

Molekulargewicht: 27kDa

Gen-ID: 598

UniProt: Q07817

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling