RBP4 Antikörper

Produktnummer ABIN6940483

Dieses Anti-RBP4-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von RBP4 in WB, IHC, IF und StM. Geeignet für Human, Maus, Ratte, Kaninchen, Affe und Ziege.

Kurzübersicht für RBP4 Antikörper (ABIN6940483)

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Reaktivität: Human, Maus, Ratte, Kaninchen, Affe, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RBP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Klon: SPM442

Produktdetails anti-RBP4 Antikörper

Spezifität: Recognizes a protein of 21 kDa-25 kDa, identified as retinol binding protein (RBP). Its epitope localizes between aa 74-182 of human RBP. This MAb recognizes reduced and carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin). RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism.

Aufreinigung: Purified by Protein A/G

Immunogen: Retinol binding protein Purified from human plasma

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Liver or placenta.

Known Application: Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu RBP4

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Andere Bezeichnung: RBP

Molekulargewicht: 21-25kDa

Gen-ID: 5947, 5948, 5950

UniProt: P02753, P09455, P50120

Pathways: Regulatorische RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response