POMC Antikörper (N-Term)

Produktnummer ABIN6940336

Der Maus Monoklonal Anti-POMC-Antikörper wurde für ELISA, IHC, FACS, IF, Coat und StM validiert. Er ist geeignet, POMC in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für POMC Antikörper (N-Term) (ABIN6940336)

Target: POMC (Proopiomelanocortin (POMC))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser POMC Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

Klon: 57

Produktdetails anti-POMC Antikörper

Bindungsspezifität: N-Term

Spezifität: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. '

Kreuzreaktivität (Details): Expected to show a broad species reactivity.

Aufreinigung: Purified by Protein A/G

Immunogen: N-terminal fragment of human ACTH conjugated to KLH

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Normal pituitary gland or pituitary tumor.

Known Application: ELISA (For coating, order Ab without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu POMC

Target: POMC (Proopiomelanocortin (POMC))

Andere Bezeichnung: POMC

Molekulargewicht: ACTH is ~5kDa, and the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

Gen-ID: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour