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Rekombinanter Cytochrome C Antikörper

Dieses Rabbit Monoclonal-Antikörper erkennt spezifisch Cytochrome C in IHC, StM. Er zeigt eine Reaktivität gegenüber Human, Rat, Mouse, Horse, Dog, Pigeon, Drosophila melanogaster, Frog.
Produktnummer ABIN6940325

Kurzübersicht für Rekombinanter Cytochrome C Antikörper (ABIN6940325)

Target

Alle Cytochrome C (CYCS) Antikörper anzeigen
Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Antikörpertyp

Recombinant Antibody

Reaktivität

  • 155
  • 118
  • 101
  • 41
  • 27
  • 21
  • 21
  • 19
  • 17
  • 15
  • 6
  • 4
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte, Maus, Pferd, Hund, Taube, Drosophila melanogaster, Frosch

Wirt

  • 96
  • 77
  • 5
  • 1
Kaninchen

Klonalität

  • 91
  • 87
  • 1
Monoklonal

Konjugat

  • 108
  • 12
  • 6
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Cytochrome C Antikörper ist unkonjugiert

Applikation

  • 140
  • 52
  • 48
  • 44
  • 36
  • 32
  • 27
  • 23
  • 22
  • 14
  • 12
  • 12
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Staining Methods (StM)

Klon

CYCS-3128R
  • Spezifität

    It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Recombinant full-length human CYCS protein

    Isotyp

    IgG
  • Applikationshinweise

    Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

    Known Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

    Andere Bezeichnung

    CYCS

    Molekulargewicht

    15kDa

    Gen-ID

    54205

    UniProt

    P99999

    Pathways

    Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity
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