Rekombinanter Cytochrome C Antikörper
Kurzübersicht für Rekombinanter Cytochrome C Antikörper (ABIN6940325)
Target
Alle Cytochrome C (CYCS) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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                                            Spezifität
- It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).
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                                            Aufreinigung
- Purified by Protein A/G
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                                            Immunogen
- Recombinant full-length human CYCS protein
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                                            Isotyp
- IgG
 
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                                            Applikationshinweise
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                        Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle. Known Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. 
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                                            Beschränkungen
- Nur für Forschungszwecke einsetzbar
 
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                                            Konzentration
- 200 μg/mL
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                                            Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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                                            Konservierungsmittel
- Sodium azide
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                                            Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            Lagerung
- 4 °C,-80 °C
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                                            Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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                                            Haltbarkeit
- 24 months
 
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    - Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
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                                            Andere Bezeichnung
- CYCS
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                                            Molekulargewicht
- 15kDa
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                                            Gen-ID
- 54205
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                                            UniProt
- P99999
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                                            Pathways
- Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity
 Target
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