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NUMA1 Antikörper

Der Maus Monoklonal Anti-NUMA1-Antikörper wurde für IHC, IF, FACS und StM validiert. Er ist geeignet, NUMA1 in Proben von Human zu detektieren.
Produktnummer ABIN6940233

Kurzübersicht für NUMA1 Antikörper (ABIN6940233)

Target

Alle NUMA1 Antikörper anzeigen
NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reaktivität

  • 86
  • 8
  • 7
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Wirt

  • 70
  • 16
Maus

Klonalität

  • 67
  • 19
Monoklonal

Konjugat

  • 48
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser NUMA1 Antikörper ist unkonjugiert

Applikation

  • 40
  • 28
  • 26
  • 26
  • 23
  • 19
  • 16
  • 15
  • 14
  • 7
  • 4
  • 1
  • 1
Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon

A73-B-D12
  • Spezifität

    Recognizes a phosphorylated protein of 228 kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200 kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

    Immunogen

    Colon carcinoma 174T cells

    Isotyp

    IgM kappa
  • Applikationshinweise

    Positive Control: Exponentially growing any cultured human cells. Tonsil or lymph node.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM citrate buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    200 μg/mL of Ab Purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

    Andere Bezeichnung

    NUMA1

    Molekulargewicht

    228kDa

    Gen-ID

    4926

    UniProt

    Q14980

    Pathways

    Caspase Kaskade in der Apoptose, Regulation of Actin Filament Polymerization, M Phase
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