NKX6-1 Antikörper

Produktnummer ABIN6940213

Der Maus Monoklonal anti-NKX6-1 Antikörper (Klon NKX61-2561) (ABIN6940213) detektiert spezifisch NKX6-1 in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für NKX6-1 Antikörper (ABIN6940213)

Target: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NKX6-1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: NKX61-2561

Produktdetails anti-NKX6-1 Antikörper

Verwendungszweck: NKX6.1 (Marker for Pancreatic and Duodenal Neuroendocrine Tumors) Antibody

Spezifität: Members of the Nkx family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart and pancreas. During neural development, sonic hedgehog (Shh) is known to control cell fate and mitogenesis, which is correlated with Shh dose-dependent expression of several genes, including Nkx-6.1. Specifically, Nkx-6.1 is responsible for cellular differentiation in the ventral neural tube and spinal meninges in response to Shh. In the pancreas, Nkx-6.1 is exclusively expressed in the islets of Langerhans in differentiating and mature B cells, which produce Insulin. The presence of Pdx-1 is required for the expression of Nkx-6.1 as well as other pancreatic B cell specific genes, including Insulin, Glut2 and IAPP. Subsequently, Nkx-6.1 binds to the DNA consensus sequence, TTAATTAC, to direct the repression of specific genes in B cells. Nkx6.1 is highly expressed in pancreatic and duodenal well-differentiated neuroendocrine tumors (WDNETS) and in metastatic WDNETS, is a highly specific marker of tumors of pancreatic origin. It has thus been suggested that Nkx6.1 is a useful inclusion into IHC panels for identifying primary sites of WDNETS.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human NKX6.1 protein

Isotyp: IgG2c, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human fetal small intestine lysate or pancreas tissue.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu NKX6-1

Target: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Andere Bezeichnung: NKX6-1

Hintergrund:

Homeobox protein NK-6 homolog A, NK homeobox, family 6, A, NK6 homeobox 1, NK6 transcription factor homolog A, NK6 transcription factor related, locus 1 (Drosophila), NKX6-1, NKX6A

Human fetal small intestine lysate or pancreas tissue.

Molekulargewicht: 40-50kDa

Gen-ID: 4825, 546270

UniProt: P78426

Pathways: Dopaminergic Neurogenesis, Positive Regulation of Peptide Hormone Secretion