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MITF Antikörper

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MITF in IHC und StM. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN6940047

Kurzübersicht für MITF Antikörper (ABIN6940047)

Target

Alle MITF Antikörper anzeigen
MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reaktivität

  • 103
  • 50
  • 25
  • 17
  • 6
  • 5
  • 5
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
Human

Wirt

  • 82
  • 39
  • 1
Maus

Klonalität

  • 70
  • 52
Monoklonal

Konjugat

  • 69
  • 7
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser MITF Antikörper ist unkonjugiert

Applikation

  • 76
  • 50
  • 42
  • 39
  • 37
  • 15
  • 13
  • 12
  • 10
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Staining Methods (StM)

Klon

D5
  • Spezifität

    MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.

    Keine Kreuzreaktivität

    Maus, Ratte (Rattus)

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    NH2 terminus fragment of human Mi protein

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    Positive Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells. Melanoma.

    Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    MITF (Microphthalmia-Associated Transcription Factor (MITF))

    Andere Bezeichnung

    MITF

    Molekulargewicht

    52-56kDa (doublet)

    Gen-ID

    4286

    UniProt

    O75030

    Pathways

    Chromatin Binding
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