Involucrin Antikörper

Produktnummer ABIN6939833

Dieses Anti-Involucrin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Involucrin in IHC, IF, FACS und StM. Geeignet für Human, Hund, Schwein, Gorilla und Owl Monkey.

Kurzübersicht für Involucrin Antikörper (ABIN6939833)

Target: Involucrin (IVL)

Reaktivität: Human, Hund, Schwein, Gorilla, Owl Monkey

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Involucrin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: SPM259

Produktdetails anti-Involucrin Antikörper

Spezifität: It recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Keine Kreuzreaktivität: Maus

Aufreinigung: Purified by Protein A/G

Immunogen: Purified involucrin from human keratinocytes

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Involucrin

Target: Involucrin (IVL)

Andere Bezeichnung: IVL

Molekulargewicht: 66-170kDa

Gen-ID: 3713

UniProt: P07476